INTERGENERIC SOMATIC HYBRID PLANTS FROM PROTOPLAST FUSION OF FORTUNELLA-CRASSIFOLIA CULTIVAR MEIWA WITH CITRUS-SINENSIS CULTIVAR VALENCIA

被引：0
|
作者：
DENG, XX [1 ]
GROSSER, JW [1 ]
GMITTER, FG [1 ]
机构：
[1] UNIV FLORIDA,INST FOOD & AGR SCI,DEPT FRUIT CROPS,CTR CITRUS RES & EDUC,LAKE ALFRED,FL 33850
来源：
SCIENTIA HORTICULTURAE | 1992年 / 49卷 / 1-2期
关键词：
CITRUS IMPROVEMENT; KUMQUAT; PLANT BREEDING; PROTOPLASTS; SWEET ORANGE;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Leaf-derived protoplasts isolated from in vitro grown seedlings of Fortunella crassifolia Swing. cultivar 'Meiwa' were fused chemically with protoplasts isolated from embryogenic suspension cultures of Citrus sinensis (L.) Osbeck cultivar 'Valencia'. Protoplasts were cultured following fusion, and mitosis was first observed after 10 days incubation in the dark at 28-degrees-C. Embryoids were recovered from regenerated calli 2 weeks after transfer to agar-solidified medium. Cytological evaluation of 27 embryoids revealed seven tetraploids and 20 diploids. Embryos were germinated and shoots dissected from germinated embryos were rooted in vitro. Counts of chromosomes in cells of young leaf meristems indicated that most regenerated plants were tetraploid (2n = 4x = 36). Peroxidase (PER) leaf isozyme banding patterns confirmed the somatic hybridity of recovered tetraploids. More than 50 somatic hybrid plants have been recovered and successfully acclimated.
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页码：55 / 62
页数：8
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