TRANSLATION OF DOUBLE-STRANDED-RNA SEGMENT-1 AND SEGMENT-2 OF CHUZAN VIRUS IN RABBIT RETICULOCYTE LYSATES

被引：0

作者：

YOSHIDA, T

HARASAWA, R

GOTO, Y

机构：

[1] MIYAZAKI UNIV, FAC AGR, DEPT VET MICROBIOL, MIYAZAKI 88921, JAPAN

[2] NATL INST ANIM HLTH, KYUSHU BRANCH LAB, KAGOSHIMA 89101, JAPAN

来源：

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 1991年 / 38卷 / 08期

关键词：

D O I：

10.1111/j.1439-0450.1991.tb00920.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Coding assignments of genome segments 1 and 2 of Chuzan virus strain K-47 were studied in vitro by using rabbit reticulocyte lysates. The double-stranded RNA segment was extracted from sodium dodecyl sulfate (SDS)-polyacrylamide gels by a modified crushing and eluting technique. Translation products labeled with [S-35]methionine were resolved by SDS-polyacrylamide gel electrophoresis. The molecular weights of the products from RNA segments 1 and 2 were estimated to be 98 and 95 kilodaltons, respectively.

引用

页码：630 / 634

页数：5

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