DETERMINATION OF FREE FATTY-ACIDS BY SUPERCRITICAL-FLUID CHROMATOGRAPHY ON AN ODS SILICA-GEL COLUMN

被引：7
|
作者：
NOMURA, A
YAMADA, J
YARITA, T
SUDO, Y
KUDO, S
NISHIZAWA, Y
机构：
[1] CHEM INSPECT & TESTING INST,TOKYO 131,JAPAN
[2] NIPPON OIL & FATS CO LTD,TSUKUBA,IBARAKI 30026,JAPAN
来源：
ANALYTICAL SCIENCES | 1995年 / 11卷 / 03期
关键词：
SUPERCRITICAL-FLUID CHROMATOGRAPHY; FREE FATTY ACID; ODS SILICA GEL COLUMN;
D O I：
10.2116/analsci.11.385
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Free fatty acids (FFAs) were determined by supercritical-fluid chromatography (SFC) on an inert ODS silica-gel column using carbon dioxide as a mobile phase without a modifier. The standard samples of FFAs with carbon numbers of 6 to 26 (even numbers) were completely separated using the pressure-programming mode. The retention behavior of FFAs was investigated in terms of the density of the mobile phase, and the FFAs were separated according to a reversed-phase mode in liquid-chromatographic terms. The composition of fatty acids of coconut oil was determined by the SFC, and was compared with gas chromatography. A good agreement was obtained. The chromatographic separation of FFAs obtained from fish oil as well as the enzymatic hydrolysis products of the fish oil were also conducted.
引用
收藏
页码：385 / 388
页数：4
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