PRECISE PHYSICAL MAPPING OF THE ESCHERICHIA-COLI RNB GENE, ENCODING RIBONUCLEASE-II

被引:11
|
作者
ZILHAO, R
CAILLET, J
REGNIER, P
ARRAIANO, CM
机构
[1] UNIV NOVA LISBOA,INST TECNOL QUIM & BIOL,P-2780 OEIRAS,PORTUGAL
[2] INST BIOL PHYSICOCHIM,F-75005 PARIS,FRANCE
来源
MOLECULAR & GENERAL GENETICS | 1995年 / 248卷 / 02期
关键词
PHYSICAL MAP; ESCHERICHIA COLI; RNB; RNA;
D O I
10.1007/BF02190807
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ribonuclease II (encoded by rnb) is one of the two main exonucleases involved in mRNA degradation in Escherichia coli. We report the precise physical mapping of rnb to 29 min on the chromosomal map in the vicinity of pyrF, and clarify the genetic and physical maps of this E. coli chromosomal region. The results were confirmed by the construction of a strain partially deleted for rnb.
引用
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页码:242 / 246
页数:5
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