PURIFICATION AND CHARACTERIZATION OF DISTINCT TYPE OF MANNOSE-SENSITIVE FIMBRIAE FROM SALMONELLA-TYPHIMURIUM

被引：0
|
作者：
GHOSH, S [1 ]
MITTAL, A [1 ]
GANGULY, NK [1 ]
机构：
[1] POSTGRAD INST MED EDUC & RES,DEPT EXPTL MED,CHANDIGARH 160012,INDIA
来源：
FEMS MICROBIOLOGY LETTERS | 1994年 / 115卷 / 2-3期
关键词：
SALMONELLA TYPHIMURIUM; FIMBRIAE; HEMAGGLUTINATION;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The fimbriae (E(4)) of a virulent strain of Salmonella typhimurium were purified by ion exchange chromatography in an FPLC system. They had a channelled appearance under transmission electron microscope and showed a major structural subunit of 17-kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The purified fimbriae were found to agglutinate guinea pig erythrocytes, but this effect was inhibited in presence of D-mannose. Immune sera raised against the Mono-Q purified fimbriae (E(4)) showed cross-reactivity with the type-1 fimbriae (F-1) composed of 21-kDa fimbrin subunit, purified by a different method from the same strain.
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页码：229 / 234
页数：6
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