THE CATALYTIC ACTIVITY OF HORSERADISH-PEROXIDASE ON LUMINOL OXIDATION IN THE REVERSED MICELLES OF SURFACTANTS IN HEPTANE

The catalytic activity of horseradish peroxidase, its conjugates with the rabbit immunoglobulins, cortisol and progesterone and the immunocomplexes of cortisol conjugate with antibodies, specific to cortisol, was compared in the luminol oxidation by hydrogen peroxide in reversed micelles of Aerosol OT and its mixtures with Triton X-45 in heptane. The chemiluminescence intensity and a time of achieving of its maximum value were varied depending on a composition and properties of micellar systems. A difference in catalytic activity of cortisol conjugates of peroxidase and their immunocomplexes with antibodies against steroids may be used for a development of homogeneous enzyme-immunoassays in reversed micelles of surfactants.