PHYSICOCHEMICAL MODELING OF SOLUTE RETENTION IN REVERSED-PHASE HPLC WITH METHANOL-PHOSPHATE BUFFER MOBILE PHASE

In this paper the new retention model primarily established for RP-HPLC systems employing methanol-water mobile phases has been adapted to the description of solute retention in analogous systems using methanol-phosphate buffer mixtures. The basic physical relationship of this approach was very carefully tested upon experimental material, and excellent agreement found between theory and practice of the phenomenon considered. This relationship can be utilized in the future for the optimization of solute retention in RP-HPLC systems with methanol-phosphate buffer mobile phases.