CD2 - A FUNCTIONAL ADHESION MOLECULE ON MURINE B-CELLS, INVOLVED IN INTERLEUKIN-4-INDUCED AGGREGATION

被引：11
|
作者：
ELENSTROMMAGNUSSON, C [1 ]
ALTEVOGT, P [1 ]
SEVERINSON, E [1 ]
机构：
[1] GERMAN CANC RES CTR, INST IMMUNOL & GENET, W-6900 HEIDELBERG 1, GERMANY
来源：
EUROPEAN JOURNAL OF IMMUNOLOGY | 1993年 / 23卷 / 04期
关键词：
CD2; ADHESION MOLECULES; INTERLEUKIN-4; B-CELLS;
D O I：
10.1002/eji.1830230418
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The murine equivalent to CD2, previously known as a T cell marker, is expressed on mouse B cells. The monoclonal anti-CD2 antibody 12-15 was found to induce B cell homotypic adhesion. When treated with F(ab')2 fragments of 12-15, purified, resting B cells aggregate within 2 h of incubation and the response is optimal after 20 h. Anti-CD2-induced aggregation is a dose-related active process, dependent on temperature, metabolic energy and divalent cations. Aggregation is inhibited by two different Fab monomers of anti-CD2, implying that it is the CD2 molecule itself that functions as an adhesion molecule.We also report that interleukin 4-induced B cell homotypic adhesion involves CD2-mediated cell binding, since the antibodies specific for mouse CD2, inhibited interleukin-4-induced cell aggregation.
引用
收藏
页码：888 / 892
页数：5
相关论文
共 50 条
  • [1] EXPRESSION AND ONTOGENY OF CD2 ON MURINE B-CELLS
    SEN, J
    ROSENBERG, N
    BURAKOFF, SJ
    [J]. JOURNAL OF IMMUNOLOGY, 1990, 144 (08): : 2925 - 2930
  • [2] A CELL-SURFACE ELISA TO DETECT INTERLEUKIN-4-INDUCED CLASS-II MHC EXPRESSION ON MURINE B-CELLS
    BARTLETT, WC
    NOELLE, RJ
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 105 (01) : 79 - 85
  • [3] A function for CD2 on murine B cells?
    Keogh, MC
    Elliot, J
    Norton, T
    Lake, RA
    [J]. IMMUNOLOGY AND CELL BIOLOGY, 1997, 75 (04): : 333 - 339
  • [4] DELETIONAL SWITCH RECOMBINATION OCCURS IN INTERLEUKIN-4-INDUCED ISOTYPE SWITCHING TO IGE EXPRESSION BY HUMAN B-CELLS
    SHAPIRA, SK
    JABARA, HH
    THIENES, CP
    AHERN, DJ
    VERCELLI, D
    GOULD, HJ
    GEHA, RS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) : 7528 - 7532
  • [5] CD2 AS A REPORTER GENE FOR TRANSIENT EXPRESSION IN ELECTROPORATED B-CELLS
    PILON, M
    LUNDGREN, E
    [J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1990, 32 (04) : 411 - 411
  • [6] MONOCLONAL-ANTIBODIES SPECIFIC FOR MURINE CD2 REVEAL ITS PRESENCE ON B-CELLS AS WELL AS T-CELLS
    YAGITA, H
    NAKAMURA, T
    KARASUYAMA, H
    OKUMURA, K
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (02) : 645 - 649
  • [7] INTERLEUKIN-4 COUNTERACTS THE INTERLEUKIN-2-INDUCED PROLIFERATION OF MONOCLONAL B-CELLS
    KARRAY, S
    DEFRANCE, T
    MERLEBERAL, H
    BANCHEREAU, J
    DEBRE, P
    GALANAUD, P
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (01): : 85 - 94
  • [8] Soluble CD21 enhances interleukin-4-induced IgE production by normal human B cells.
    Frémeaux-Bacchi, V
    Peyramaure, S
    Rakotobe, S
    Fischer, EM
    Kazatchkine, MD
    [J]. MOLECULAR IMMUNOLOGY, 1998, 35 (6-7) : 347 - 347
  • [9] CD23 FUNCTIONS AS AN ADHESION MOLECULE INVOLVED IN HOMOTYPIC AGGREGATION OF HUMAN LYMPHOCYTES-B
    BJORCK, P
    ELENSTROM, C
    SEVERINSON, E
    ROSEN, A
    PAULIE, S
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, : 167 - 167
  • [10] INTERLEUKIN-2 DOES NOT INDUCE MURINE B-CELLS TO SECRETE IG
    PURE, E
    ISAKSON, PC
    PAETKAU, V
    CAPLAN, B
    VITETTA, ES
    KRAMMER, PH
    [J]. JOURNAL OF IMMUNOLOGY, 1982, 129 (06): : 2420 - 2425
12345