ENHANCEMENT OF GAP JUNCTIONAL INTERCELLULAR COMMUNICATION IN TUMOR PROMOTER-TREATED CELLS BY COMPONENTS OF GREEN TEA

被引:62
|
作者
SIGLER, K [1 ]
RUCH, RJ [1 ]
机构
[1] MED COLL OHIO, DEPT PATHOL, HEB 202, 3000 ARLINGTON AVE, TOLEDO, OH 43699 USA
关键词
GREEN TEA; CATECHINS; TUMOR PROMOTION; CARCINOGENESIS; GAP JUNCTIONS; CHEMOPREVENTION;
D O I
10.1016/0304-3835(93)90026-6
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Green tea (Camellia sinensis) has been reported to inhibit tumor promotion in vivo and in vitro. Many tumor promoters inhibit gap junctional intercellular communication (GJIC) which may be an important mechanism of promotion. In the present study, we hypothesized that green tea would enhance GJIC in promoter-treated cells. An aqueous extract of green tea (GTE) and several of its constituents were tested for their effects on GJIC in p,p'-dichlorodiphenyltrichloroethane (DDT)-, 12-O-tetradecanoylphorbol-13-acetate (TPA)- and dieldrin-treated WB-F344 rat liver epithelial cells. All three promoters inhibited GJIC in a dose-responsive manner at non-cytolethal concentrations. GTE (10-80 gamma/ml) enhanced GJIC 20-80% in promoter-treated cells. (-)-Epigallocatechin gallate and (-)-epicatechin gallate also enhanced GJIC in DDT-treated cells, but no effects were seen with (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, caffeine, or theobromine. These data suggest GTE may inhibit tumor promotion by enhancing GJIC and that the most active components are the catechin gallates.
引用
收藏
页码:15 / 19
页数:5
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