SPECIFIC PROLINE AND HYDROXYPROLINE DETECTION METHOD BY POSTCOLUMN DERIVATIZATION FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：10
|
作者：
OZAKI, A [1 ]
SHIBASAKI, T [1 ]
MORI, H [1 ]
机构：
[1] KYOWA HAKKO KOGYO CO LTD, TOKYO RES LABS, MACHIDA 194, TOKYO, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 09期
关键词：
D O I：
10.1271/bbb.59.1764
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A specific detection method for hydroxyproline by post-column derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole was developed after chiral resolution by ligand-exchange chromatography, The addition of ethylenediaminetetraacetic acid to the reaction mixture was effective to achieve the post-column reaction, The calibration curve for trans-4-hydroxy-L-proline shows linearity from 7.6 pmol up to 7600 pmol.
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页码：1764 / 1765
页数：2
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