HIGH-YIELD PURIFICATION OF GLUCOKINASE FROM RAT-LIVER

被引：11
|
作者：
MIWA, I
MITSUYAMA, S
TOYODA, Y
MURATA, T
OKUDA, J
机构：
[1] Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University., Nagoya
来源：
PREPARATIVE BIOCHEMISTRY | 1990年 / 20卷 / 02期
关键词：
D O I：
10.1080/00327489008050187
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid and reliable method for the purification of rat liver glucokinase was developed. The procedure consists of DEAE-cellulose ion-exchange chromatography, Phenyl-Sepharose hydrophobic interaction chromatography, DEAE-Affi Gel Blue dye-ligand chromatography, and duplicate steps of glucosamine-Sepharose affinity chromatography. Glucokinase was purified to a specific activity of 290 units/mg protein in a yield of 55% in 6 days. The final enzyme preparations were completely homogeneous in most experiments as assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The estimated molecular weight (51,000) and sigmoidal saturation function for glucose of purified glucokinase were in good agreement with published data. © 1990, Taylor & Francis Group, LLC. All rights reserved.
引用
收藏
页码：163 / 178
页数：16
相关论文
共 50 条
  • [1] PURIFICATION IN HIGH-YIELD AND CHARACTERIZATION OF RAT HEPATIC GLUCOKINASE
    HOLROYDE, MJ
    ALLEN, MB
    STORER, AC
    WARSY, AS
    CHESHER, JME
    TRAYER, IP
    CORNISHBOWDEN, A
    WALKER, DG
    BIOCHEMICAL JOURNAL, 1976, 153 (02) : 363 - +
  • [2] PURIFICATION OF RAT-LIVER GLUCOKINASE
    TOYODA, Y
    MIWA, I
    KAMIYA, M
    OGISO, S
    OKUDA, J
    PREPARATIVE BIOCHEMISTRY, 1994, 24 (3-4): : 225 - 236
  • [3] A NEW HIGH-YIELD PROCEDURE FOR THE PURIFICATION OF THE NONSPECIFIC PHOSPHOLIPID TRANSFER PROTEIN FROM RAT-LIVER
    POORTHUIS, BJHM
    GLATZ, JFC
    AKEROYD, R
    WIRTZ, KWA
    BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 665 (02) : 256 - 261
  • [4] AN IMPROVED PROCEDURE FOR THE SIMULTANEOUS PURIFICATION IN HIGH-YIELD OF PEROXISOMES, LYSOSOMES, AND MITOCHONDRIA FROM RAT-LIVER
    SEPHTON, GB
    BUECHLER, KF
    LOWENSTEIN, JM
    PROTOPLASMA, 1990, 153 (03) : 127 - 135
  • [5] HIGH-YIELD BACTERIAL EXPRESSION, PURIFICATION, AND FUNCTIONAL RECONSTITUTION OF THE TRICARBOXYLATE TRANSPORT PROTEIN FROM RAT-LIVER MITOCHONDRIA
    XU, Y
    MAYOR, JA
    GREMSE, D
    WOOD, DO
    KAPLAN, RS
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 207 (02) : 783 - 789
  • [6] HIGH-YIELD PREPARATIVE METHOD FOR ISOLATION OF RAT-LIVER MITOCHONDRIA
    BUSTAMANTE, E
    SOPER, JW
    PEDERSEN, PL
    ANALYTICAL BIOCHEMISTRY, 1977, 80 (02) : 401 - 408
  • [7] PREPARATION OF RAT-LIVER PLASMA-MEMBRANES IN A HIGH-YIELD
    LOTEN, EG
    REDSHAWLOTEN, JC
    ANALYTICAL BIOCHEMISTRY, 1986, 154 (01) : 183 - 185
  • [8] PURIFICATION OF GLUCOKINASE FROM RAT LIVER
    BABUL, J
    NIEMEYER, H
    ARCHIVOS DE BIOLOGIA Y MEDICINA EXPERIMENTALES, 1966, 3 (2-3): : 65 - &
  • [9] RAPID, HIGH-YIELD PURIFICATION OF RAT-LIVER GLUTATHIONE-PEROXIDASE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    LYONS, DE
    WILHELMSEN, EC
    TAPPEL, AL
    JOURNAL OF LIQUID CHROMATOGRAPHY, 1981, 4 (11): : 2063 - 2071
  • [10] RAPID, HIGH-YIELD PURIFICATION OF RAT-LIVER 3-HYDROXY-3-METHYL-GLUTARYL-COENZYME-A REDUCTASE
    ROGERS, DH
    PANINI, SR
    RUDNEY, H
    ANALYTICAL BIOCHEMISTRY, 1980, 101 (01) : 107 - 111
12345