INDUCIBLE EXPRESSION OF PATHOGENESIS-RELATED 1-PROTEIN GENE IN TRANSFORMED PLANTS

被引：0
|
作者：
OHASHI, Y
OHSHIMA, M
MATSUOKA, M
机构：
来源：
JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY | 1990年 / 24卷 / 02期
关键词：
AGROBACTERIUM INFECTION; ELECTROPORATION; NICOTIANA-TABACUM; STRESS PROTEIN; TRANSFORMATION;
D O I：
暂无
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Induced expression of tobacco pathogenesis-related (PR) 1 protein genes was analyzed. The base sequence comparision of the active three genes (PR1a, PR1b and PR1c genes) indicated that there was a well conserved region in 5' flanking 180 nucleotides in their promotor sequences. Direct gene transfer into tobacco protoplasts by electroporation with the chimeric gene of PR1a promoter and a reporter gene (beta-glucuronidase: GUS), and stable transformation of tobacco with a binary vector mediated Agrobacterium infection with the same chimeric gene suggested that the cis-acting element responding to stress or salicylic acid be present in the 0.3Kb sequence of the 5' flanking region of PR1a gene.
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页码：91 / 99
页数：9
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