NUCLEOTIDE-SEQUENCE OF A CDNA FOR MOUSE SQUALENE EPOXIDASE

被引：21
|
作者：
KOSUGA, K
HATA, S
OSUMI, T
SAKAKIBARA, J
ONO, T
机构：
[1] HIMEJI INST TECHNOL,FAC SCI,DEPT LIFE SCI,HIMEJI,HYOGO 67812,JAPAN
[2] NIIGATA UNIV,SCH MED,DEPT BIOCHEM,NIIGATA 951,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1260卷 / 03期
关键词：
SQUALENE EPOXIDASE; CHOLESTEROL BIOSYNTHESIS; CDNA CLONING; FAD-BINDING MOTIF; (MOUSE LIVER);
D O I：
10.1016/0167-4781(94)00245-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A full-length cDNA encoding mouse squalene epoxidase was isolated by screening a mouse liver cDNA library with the rat squalene epoxidase gene as a probe. The cDNA had an open reading frame for a 572 amino acid polypeptide with a calculated molecular mass of 63.8 kDa. The predicted amino acid sequence of the mouse enzyme contained an FAD-binding motif, and was 93% identical to that of the rat enzyme. The former is one amino acid shorter than the latter. Blotting analyses showed that the mRNA is 2.8 kb in size and that a single copy of the gene is present in the mouse genome.
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页码：345 / 348
页数：4
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