CHARACTERIZATION OF THE OPISTHORCHIS-VIVERRINI GENOME

被引：5
|
作者：
SERMSWAN, R
MONGKOLSUK, S
SIRISINHA, S
机构：
[1] MAHIDOL UNIV,FAC SCI,DEPT MICROBIOL,RAMA 6 RD,BANGKOK 10400,THAILAND
[2] MAHIDOL UNIV,FAC SCI,DEPT BIOTECHNOL,BANGKOK 10400,THAILAND
来源：
JOURNAL OF HELMINTHOLOGY | 1991年 / 65卷 / 01期
基金：
英国惠康基金;
关键词：
OPISTHORCHIS-VIVERRINI; GENOME; DNA; RESTRICTION ENZYMES;
D O I：
10.1017/S0022149X00010439
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The methylations of trematode genomic DNA were analyzed using restriction enzymes and Southern blot hybridization. Restriction enzymes MspI, HpaII, HhaI were used to probe CpG methylation while MboI, Sau3A, DpnI were used for A methylation. The results revealed that Opisthorchis viverrini, Fasciola gigantica and Gigantocotyle siamensis had neither CpG nor A methylations. The presence of highly repeated DNA elements was also demonstrated in O. viverrini genomic DNA.
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收藏
页码：51 / 54
页数：4
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