SEPARATION OF OUTER CYSTS FROM METACERCARIAE OF FASCIOLA-GIGANTICA IN AN AQUEOUS 2-PHASE SYSTEM

被引：2
|
作者：
YOSHIHARA, S
HAZEYAMA, M
KATO, H
GOTO, N
机构：
[1] NATL INST ANIM HLTH,KYUSHU BRANCH LAB,KAGOSHIMA 89101,JAPAN
[2] NATL INST HLTH,DEPT SAFETY RES BIOL,TOKYO 208,JAPAN
来源：
JOURNAL OF HELMINTHOLOGY | 1995年 / 69卷 / 01期
关键词：
D O I：
10.1017/S0022149X00013882
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The outer cysts (Oc) of Fasciola gigantica metacercariae (Mc) were separated in an aqueous two-phase system composed of polyethylene glycol and dextran in distilled water A large number of separated Mc without Oc were recovered in the bottom layer rich in dextran in the system. Ten separated Mc in the layer were used to inoculate the mouse with a syringe and juveniles were recovered from the animals on the 14th day. When the number of juveniles recovered was compared with that from the mice infected orally with the same number of Mc, there was a significant difference between them (P <0.05). The former method is easier than the latter for inoculation.
引用
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页码：85 / 87
页数：3
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