PREPARATION OF ASTATINE-LABELED MONOCLONAL-ANTIBODIES

被引：0
|
作者：
MILESZ, S [1 ]
NORSEEV, YV [1 ]
SZUCS, Z [1 ]
VASAROS, L [1 ]
机构：
[1] CENT INST PHYS RES,BUDAPEST,HUNGARY
来源：
RADIOCHEMISTRY | 1995年 / 37卷 / 03期
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D O I：
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中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In the cationic state astatine forms a stable complex with diethylenetriaminepentaacetic acid. Thanks to this complex, astatine can be bound to monoclonal antibodies of the RYa(1) type. The most favorable conditions for preparing astatine-labeled antibodies are established. The chromatographic analysis and electromigration experiments showed that astatine is firmly linked to a biomolecule in vitro and it did not escape from labeled monoclonal antibodies even under treatment with such highly effective astatine-complexing agent as thiourea. The immune activity of astatine-labeled antibodies did not change even after 20 h.
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页码：233 / 237
页数：5
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