ISOLATION OF BABESIA-BIGEMINA AND BABESIA-BOVIS MEROZOITES BY AMMONIUM-CHLORIDE LYSIS OF INFECTED ERYTHROCYTES

被引:0
|
作者
MACHADO, RZ [1 ]
VALADAO, CAA [1 ]
MELO, WR [1 ]
ALESSI, AC [1 ]
机构
[1] UNIV SAO PAULO,FAC MED,DEPT MORFOL,BR-14049904 RIBEIRAO PRET,BRAZIL
关键词
BABESIA BOVIS; BABESIA BIGEMINA; MEROZOITE ISOLATION;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
1. We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.
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页码:2591 / 2598
页数:8
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