METHODS FOR SELECTION OF LOW-TEMPERATURE TOLERANT MUTANTS OF CHRYSANTHEMUM-MORIFOLIUM RAMAT USING IRRADIATED CELL-SUSPENSION CULTURES .2. PRESELECTION INVITRO UNDER LOW-TEMPERATURE STRESS

被引：7
|
作者：
PREIL, W
HUITEMA, JBM
DEJONG, J
机构：
[1] AGR UNIV WAGENINGEN,DEPT PLANT PHYSIOL,6703 BD WAGENINGEN,NETHERLANDS
[2] CTR PLANT BREEDING RES,6700 AA WAGENINGEN,NETHERLANDS
来源：
PLANT BREEDING | 1991年 / 107卷 / 02期
关键词：
CHRYSANTHEMUM-MORIFOLIUM; LOW-TEMPERATURE TOLERANCE; CELL SUSPENSION CULTURE; REGENERATION; IRRADIATION; MUTANTS;
D O I：
10.1111/j.1439-0523.1991.tb00540.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The effect of in vitro cold treatment (+6-degrees-C, 90 days) on selection of low-temperature tolerant (LTT) mutants was investigated using X-irradiated (15 Gy and 20 Gy) and non-irradiated cell suspensions of Chrysanthemum morifolium cv. 'Parliament'. Low temperature reduced significantly the number of regenerating calli, especially in the irradiated cultures. After the end of the cold stress 684, 197 and 54 plants were obtained from the O Gy, 15 Gy and 20 Gy treated cultures, respectively. Testing these plants in the greenhouse (+12-degrees-C, winter low-light) most of the early flowering variants were selected from the 15 Gy population.
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页码：131 / 134
页数：4
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