APPLICATION OF CAPILLARY ZONE ELECTROPHORESIS TO THE CHARACTERIZATION OF MULTIPLE ANTIGEN PEPTIDES

被引：17
|
作者：
PESSI, A
BIANCHI, E
CHIAPPINELLI, L
NARDI, A
FANALI, S
机构：
[1] CNR,IST CROMATOG,AREA RIC ROMA,CP 10,I-00016 MONTEROTONDO,ITALY
[2] SCLAVO SPA,PEPTIDE SYNTH UNIT,I-00015 MONTEROTONDO,ITALY
来源：
JOURNAL OF CHROMATOGRAPHY | 1991年 / 557卷 / 1-2期
关键词：
D O I：
10.1016/S0021-9673(01)87140-5
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Capillary zone electrophoresis (CZE) was applied to the analysis of a recently described class of synthetic branched peptides, multiple antigen peptides (MAPs). In comparison with high-performance liquid chromatography (HPLC), CZE showed superior resolution of minor impurities in samples of MAPs obtained by preparative chromatography. MAPs that differed only in the substitution of uncharged residues (Ala-Ser) could be separated by the addition of organic solvents (acetonitrile, methanol, ethanol) to the aqueous background electrolyte.
引用
收藏
页码：307 / 313
页数：7
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