OPTIMIZATION OF NAFION-COATED ELECTRODES FOR SELECTIVE DETECTION IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The performance of Nafion-coated electrodes for detection in liquid chromatography was optimized. The effect of the composition of the mobile phase and the film thickness on the selectivity of the detection of catecholamines was investigated. The stability and the response of the coated electrodes were also determined. From the results, general guidelines were formulated for the optimum use of Nafion-coated electrodes in high-performance liquid chromatography. The increase in the selectivity when using the Nafion-coated electrode was demonstrated by analyzing urine samples.