MOLECULAR MOTORS ARE DIFFERENTIALLY DISTRIBUTED ON GOLGI MEMBRANES FROM POLARIZED EPITHELIAL-CELLS

被引:180
|
作者
FATH, KR [1 ]
TRIMBUR, GM [1 ]
BURGESS, DR [1 ]
机构
[1] UNIV PITTSBURGH, DEPT BIOL SCI, PITTSBURGH, PA 15260 USA
来源
JOURNAL OF CELL BIOLOGY | 1994年 / 126卷 / 03期
关键词
D O I
10.1083/jcb.126.3.661
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Microtubules (MT) are required for the efficient transport of membranes from the trans-Golgi and for transcytosis of vesicles from the basolateral membrane to the apical cytoplasm in polarized epithelia. MTs in these cells are primarily oriented with their plus ends basally near the Golgi and their minus-ends in the apical cytoplasm. Here we report that isolated Golgi and Golgi-enriched membranes from intestinal epithelial cells possess the actin based motor myosin-I, the MT minus-end-directed motor cytoplasmic dynein and its in vitro motility activator dynactin (p150/Glued). The Golgi can be separated into stacks, possessing features of the Golgi cisternae, and small membranes enriched in the trans-Golgi network marker TGN 38/41. Whereas myosin-I is present on all membranes in the Golgi fraction, dynein is present only on the small membrane fraction. Dynein, like myosin-I, is associated with membranes as a cytoplasmic peripheral membrane protein. Dynein and myosin-I coassociate with membranes that bind to MTs and cross-link actin filaments and MTs in a nucleotide-dependent manner. We propose that cytoplasmic dynein moves Golgi membranes along MTs to the cell cortex where myosin-I provides local delivery through the actin-rich cytoskeleton to the apical membrane.
引用
收藏
页码:661 / 675
页数:15
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