PHYSIOLOGICAL OXYGEN-TENSION IS RELEVANT TO MHC-1 EXPRESSION, SPONTANEOUS TRANSFORMATION, AND INTERFERON RESPONSE OF IN-VITRO AGING MURINE FIBROBLASTS

被引：10

作者：

EBBESEN, P ^{[1
]}

HAGER, H ^{[1
]}

ABOAGYEMATHIESEN, G ^{[1
]}

PETERSEN, PM ^{[1
]}

LUTZHOFT, J ^{[1
]}

VILLADSEN, JA ^{[1
]}

ZDRAVKOVIC, M ^{[1
]}

DALSGAARD, AM ^{[1
]}

ZACHAR, V ^{[1
]}

机构：

[1] SLOVAK ACAD SCI, INST VIROL, CS-80936 BRATISLAVA, CZECHOSLOVAKIA

来源：

EXPERIMENTAL GERONTOLOGY | 1993年 / 28卷 / 06期

基金：

英国医学研究理事会;

关键词：

OXYGEN TENSION; IN-VITRO AGING; PRIMARY CULTURE; IN-VITRO TRANSFORMATION; INTERFERON; MAJOR HISTOCOMPATIBILITY COMPLEX;

D O I：

10.1016/0531-5565(93)90046-G

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Working from the hypothesis that modest deviations from physiological oxygen tension will influence cell characteristics important for infections/immunity and tumor development, cells were studied at three oxygen tensions during in vitro aging. Primary mouse embryo fibroblasts were established and subsequently passaged at 3, 6, and 18 kPa oxygen tension (6 representing normal tissue tension and 18 being the conventionally tension at in vitro cultures). The growth rate was slightly higher at 6 than 3 and 18 kPa. All cultures eventually stopped growing and subsequently transformed to nonmalignant cells with unlimited growth capacity. Cells kept at 3 kPa reached the highest number of cell doublings before crisis. Stimulation with PolyI:C resulted in detectable interferon response only at the high oxygen tension, and after transformation none of the cultures responded with interferon production. Expression of the major histocompatibility complex H-2K was elevated above and below physiological oxygen tension, indicating regulatory processes optimizing MHC expression at about physiological oxygen tension.

引用

页码：573 / 578

页数：6