IMMOBILIZED CELLULOLYTIC AND HEMICELLULOLYTIC ENZYMES FROM MACROPHOMINA-PHASEOLINA

Cellulolytic enzymes from M. phaseolina were immobilized in acrylamide polymer. The immobilized enzyme preparation showed activity towards filter paper and cotton. The degree of hydrolysis of highly organized cellulose, particularly cotton, appears to be low in comparison with that of soluble substrate. The kinetic studies of immobilized enzymes indicated the presence of diffusional limitations by the increase in Vmax as the particle size decreased. The operational studies suggested that the immobilized enzymes retained the original activities up to 25-29 times in the reuse cycle.