CHARACTERIZATION OF A RHIZOBIUM-ETLI CHROMOSOMAL GENE REQUIRED FOR NODULE DEVELOPMENT ON PHASEOLUS-VULGARIS L

被引：7
|
作者：
POOYAN, S [1 ]
GEORGE, MLC [1 ]
BORTHAKUR, D [1 ]
机构：
[1] UNIV HAWAII,DEPT PLANT MOLEC PHYSIOL,3050 MAILE WAY,GIMORE 402,HONOLULU,HI 96822
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1994年 / 10卷 / 05期
关键词：
MOTILITY; NDV GENE; NITROGEN FIXATION; NODULATION; RHIZOBIUM;
D O I：
10.1007/BF00367673
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chromosomal fragment. The 3.6-kb fragment was subcloned from the cosmid pUHR80 which complemented MLC640. Further subcloning and site-directed Tn5 mutagenesis localized the gene for nodule development to a 1.7-kb region within the 3.6-kb EcoRI fragment. Southern hybridization using the 3.6-kb EcoRI fragment as the probe against genomic DNA of several Rhizobium spp. indicated that this gene is conserved in different rhizobia.
引用
收藏
页码：583 / 589
页数：7
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