A ZYMOGRAM METHOD TO DETECT ENDOGLUCANASES FROM BACILLUS-SUBTILIS, MYROTHECIUM-VERRUCARIA AND TRICHODERMA-REESEI

被引：11

作者：

HOLT, SM ^{[1
]}

HARTMAN, PA ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT MIROBIOL IMMUNOL & PREVENT MED,205 SCI 1,AMES,IA 50011

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1994年 / 13卷 / 01期

关键词：

BACILLUS-SUBTILIS; MYROTHECIUM-VERRUCARIA; TRICHODERMA-REESEI; ENDOGLUCANASE;

D O I：

10.1007/BF01569654

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A purified endoglucanase (Eg) from Bacillus subtilis and crude Eg preparations from Myrothecium verrucaria and Trichoderma reesei were denatured by heat in the presence of SDS and separated by using SDS polyacrylamide gel electrophoresis. Eg zymogram activity bands were detected directly in carboxymethylcellulose-laden separating gels after enzyme renaturation. Denaturing zymogram analysis was a simple method for the separation and detection of Egs.

引用

页码：2 / 4

页数：3

共 8 条

[1] DIFFERENTIAL EXPRESSION OF XYLANASES AND ENDOGLUCANASES IN THE HYBRID DERIVED FROM INTERGENETIC PROTOPLAST FUSION BETWEEN A CELLULOMONAS SP AND BACILLUS-SUBTILIS
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[8] KINETIC STUDIES BY STOPPED-FLOW METHOD OF HYDROLYSIS OF ORTHO-NITROPHENYL ALPHA-MALTOSIDE CATALYZED BY SACCHARIFYING ALPHA-AMYLASE FROM BACILLUS-SUBTILIS
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