NUCLEOTIDE-SEQUENCE OF CDNA-ENCODING ZP3-ALPHA, A SPERM-BINDING GLYCOPROTEIN FROM ZONA-PELLUCIDA OF PIG OOCYTE

被引：86
|
作者：
YUREWICZ, EC [1 ]
HIBLER, D [1 ]
FONTENOT, GK [1 ]
SACCO, AG [1 ]
HARRIS, J [1 ]
机构：
[1] ZONAGEN,THE WOODLANDS,TX
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1174卷 / 02期
关键词：
ZONA-PELLUCIDA; CDNA; OOCYTE; SPERM-BINDING PROTEIN; CDNA SEQUENCE; NUCLEOTIDE SEQUENCE; (PIG);
D O I：
10.1016/0167-4781(93)90119-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We isolated a cDNA encoding the pig oocyte zona pellucida protein ZP3alpha from a pig ovary lambdagt11 cDNA library. The 1699 bp cDNA contains a short 3' untranslated region characteristic of cDNAs encoding zona proteins. The deduced amino acid sequence for ZP3alpha consists of 536 amino acid residues and shares 66% overall identity with a 55 kDa rabbit zona protein. Important features of the ZP3alpha polypeptide include a predicted N-terminal signal sequence, twenty-two cysteine residues, an 0-glycosylated domain and potential attachment sites for five N-linked sugar chains. A multibasic tetrapeptide occurs upstream of a predicted C-terminal transmembrane sequence; this suggests proteolytic processing of an integral membrane precursor within the constitutive secretory pathway.
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页码：211 / 214
页数：4
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