EXPRESSION OF THE RETINOBLASTOMA GENE-PRODUCT IN HUMAN HEPATOCELLULAR-CARCINOMA

Using the mouse anti-human retinoblastoma gene product (pRB) monoclonal antibody, PMG3-245, pRB was detected immuno-histocytochemically in human hepatocellular carcinoma (HCC) tissues and a human HCC cell line, designated OCUH-16, recently established in our laboratory. This antibody reacted with human pRB and yielded a single band of approximately 110 kd from cultured OCUH-16 cells. The granules that stained for pRB were found mostly in the HCC cell nuclei, with a few granules observed in the rough endoplasmic reticulum by electron microscopy. Most of the stained granules were located in the euchromatin-rich areas. The percentage of OCUH-16 cells that expressed pRB or DNA polymerase alpha (DNA-PA) decreased over time as the number of OCUH-16 cells increased. The number of HCC cells that stained for pRB in the biopsy specimens from 11 patients varied and pRB expression was well maintained in early and advanced HCC. The level of pRB expression did not correlate with the differentiation of HCC cells or the clinical prognosis. The expression of pRB statistically correlated with that of DNA-PA (P < .01; r = .92). Some sinusoidal cells also stained for PRE. These findings imply that large deletions in the pRB gene are rare in the initiation or promotion of HCC. The correlation between PRE and DNA-PA may suggest that stained pRB participates in the proliferation of both HCC and non-HCC cells. HUM PATHOL 26:366-374. Copyright (C) 1995 by W.B. Saunders Company