A RAPID, TETRAZOLIUM-BASED ASSAY FOR TOXICITY OF THE PHYTOALEXIN DESOXYHEMIGOSSYPOL TO VERTICILLIUM-DAHLIAE

被引：2
|
作者：
MACE, ME [1 ]
ELISSALDE, MH [1 ]
STIPANOVIC, RD [1 ]
BELL, AA [1 ]
机构：
[1] USDA ARS,ENTOMOL RES LABS,COTTON PATHOL RES UNIT,COLLEGE STN,TX 77840
来源：
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 1990年 / 38卷 / 01期
关键词：
D O I：
10.1016/0048-3575(90)90148-U
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid, tetrazolium-based assay was developed for measuring the toxicity of the terpenoid phytoalexin desoxyhemigossypol (dHG) to the cotton wilt pathogen Verticillium dahliae. The assay is based on the enzymatic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) by live Verticillium dahliae. The absorption of the reduced blue formazan product of MTT is read in multiwell plates with an ELISA reader. This rapid, quantitative assay yields a dose-response curve that allows examination of the toxicity of dHG to numerous stains of V. dahliae which is impossible with nonquantitative, solid media-based assays. © 1990.
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页码：57 / 59
页数：3
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