FLUORESCEIN DIACETATE UPTAKE TO DETERMINE THE VIABILITY OF HUMAN FETAL CEREBRAL CORTICAL-CELLS

被引：4

作者：

ZHANG, F ^{[1
]}

ZHANG, PY ^{[1
]}

WANG, EM ^{[1
]}

SU, FZ ^{[1
]}

QI, YC ^{[1
]}

BU, X ^{[1
]}

WANG, YF ^{[1
]}

机构：

[1] HENAN TUMOR INST,ZHENGZHOU,PEOPLES R CHINA

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1995年 / 70卷 / 04期

关键词：

FETAL TISSUE; CEREBRUM; CORTEX; VIABILITY; FLUORESCEIN DIACETATE; TRYPAN BLUE;

D O I：

10.3109/10520299509107310

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated the accuracy of fluorescein diacetate uptake as an indicator of the viability of human fetal cerebral cortical cells. Cortical cells from 16-26-week-old normal fetuses were studied, The cortices were dissociated mechanically with normal saline to make a suspension. Fluorescein diacetate uptake and trypan blue exclusion were compared as methods for examining cell viability. Our results show that fluorescein diacetate uptake is a simple and sensitive method for examining human fetal cortical cell viability.

引用

页码：185 / 187

页数：3

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