PHOSPHORYLATION OF ORNITHINE DECARBOXYLASE IN INTACT ERYTHROLEUKEMIA-CELLS

被引：14
|
作者：
FLAMIGNI, F
MARMIROLI, S
MEGGIO, F
GUARNIERI, C
PINNA, LA
机构：
[1] UNIV PADUA,DEPARTIMENTO CHIM BIOL,VIA TRIESTE 75,I-35131 PADUA,ITALY
[2] UNIV BOLOGNA,DEPARTIMENTO CHIM,I-40126 BOLOGNA,ITALY
[3] IST RIZZOLI,CNR,IST CITOMORFOL,BOLOGNA,ITALY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1052卷 / 02期
关键词：
(Erythroleukemia cell); Ornithine decarboxylase; Protein phosphorylation;
D O I：
10.1016/0167-4889(90)90232-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
32P-labeled ornithine decarboxylase was isolated by immunoprecipitation from murine erythroleukemia cells incubated in a medium containing [32P]ortophosphoric acid. Analysis of immunoprecipitate by SDS-polyacrylamide gel electrophoresis and autoradiography revealed a radiolabeled band, which corresponded to the position of mouse ornithine decarboxylase, phosphorylated in vitro by casein kinase-2. A preparation of casein kinase-2 purified from nuclei of erythroleukemia cells could also phosphorylate mouse ornithine decarboxylase. © 1990.
引用
收藏
页码：345 / 347
页数：3
相关论文
共 50 条
12345