A DNA TOPOISOMERASE-I INHIBITOR BLOCKS THE DIFFERENTIATION OF RAT GRANULOSA-CELLS INDUCED BY FOLLICLE-STIMULATING-HORMONE

被引：8
|
作者：
BARANAO, JL
BLEY, MA
BATISTA, FD
GLIKIN, GC
机构：
[1] UNIV BUENOS AIRES, FAC CIENCIAS EXACTAS & NAT, RA-1428 BUENOS AIRES, ARGENTINA
[2] UNIV BUENOS AIRES, CONSEJO NACL INVEST CIENT & TECN, RA-1428 BUENOS AIRES, ARGENTINA
来源：
BIOCHEMICAL JOURNAL | 1991年 / 277卷
关键词：
D O I：
10.1042/bj2770557
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Follicle-stimulating hormone (FSH), acting through a cyclic AMP-mediated mechanism, promotes differentiation of rat granulosa cells cultured in a defined medium. Camptothecin, a DNA topoisomerase I blocker, inhibited the increase in progesterone and oestradiol production stimulated by FSH. This effect was not due to non-specific inhibition of protein synthesis, as shown by measurement of [S-35]methionine incorporation. A transient increase in DNA topoisomerase I activity was observed after 24 h of culture in the presence of FSH or dibutyryl cyclic AMP. Our results are consistent with a key role for DNA topoisomerase I in the modulation of gene expression by FSH in rat granulosa cells.
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页码：557 / 560
页数：4
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