PRODUCTION OF CELL WALL-DEGRADING ENZYMES BY THE PHYTOPATHOGENIC FUNGUS SCLEROTINIA-SCLEROTIORUM

被引:124
|
作者
RIOU, C
FREYSSINET, G
FEVRE, M
机构
[1] UNIV LYON 1, BIOL CELLULAIRE FONG LAB,CNRS,UMR 106,43 BD, BD 11 NOVEMBRE 1918, F-69622 VILLEURBANNE, FRANCE
[2] RHONE POULENC AGROCHIM, SERV BIOL MOLEC & CELLULAIRE VEGETALES, F-69263 Lyon 09, FRANCE
关键词
D O I
10.1128/AEM.57.5.1478-1484.1991
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The range of polysaccharide-degrading enzymes and glycosidases formed by the phytopathogenic fungus Sclerotinia sclerotiorum was monitored following growth on 16 carbohydrate substrates. Endo- and exoenzymes capable of degrading cellulosic, hemicellulosic, and pectinolytic polysaccharides were secreted. Pectinolytic activities were produced constitutively on all of the substrates tested. Cellulolytic enzymes were not induced in simple sugar (i.e., glucose or xylose) media. Polysaccharide growth substrates and cellulase inducers increased all of the enzyme activities tested. Gel filtration analysis revealed the appearance of new molecular forms of pectinase, beta-xylosidase, and cellobiosidase during induction on pectin and carboxymethyl cellulose media.
引用
收藏
页码:1478 / 1484
页数:7
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