COPPER-ZINC SUPEROXIDE-DISMUTASE FROM ASCARIS-SUUM (NEMATODA) - PURIFICATION AND CHARACTERIZATION

被引：1
|
作者：
SANCHEZMORENO, M [1 ]
ENTRALA, E [1 ]
JANSSEN, D [1 ]
OSUNA, A [1 ]
机构：
[1] UNIV GRANADA,FAC SCI,MOLEC PARASITOL LAB,E-18071 GRANADA,SPAIN
来源：
BIOSCIENCE REPORTS | 1994年 / 14卷 / 02期
关键词：
COPPER-ZINC SUPEROXIDE DISMUTASE; ASCARIS SUUM; PURIFICATION AND CHARACTERIZATION;
D O I：
10.1007/BF01210303
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Copper-zinc superoxide dismutase from Ascaris suum (Nematoda) was purified in a new, more efficient. and Easter manner. The process included differential centrifugation, fractionation with ammonium sulfate, and sodium dodecyl sulfate-polyacrylamide electrophoresis, yielding a 340-fold purification (specific activity of 47 units/mg). Optimal storage conditions, optimal pH range, thermostability, molecular weight and ultravioltet-visible absorption spectrum of the enzyme are described, and a new enzymatic model for pharmacological screening is suggested.
引用
收藏
页码：83 / 90
页数：8
相关论文
共 50 条
  • [1] SUPEROXIDE-DISMUTASE FROM ASCARIS-SUUM
    SANCHEZMORENO, M
    MONTEOLIVA, M
    FATOU, A
    GARCIARUIZ, MA
    [J]. PARASITOLOGY, 1988, 97 : 345 - 353
  • [2] PHYSICOCHEMICAL CHARACTERISTICS OF SUPEROXIDE-DISMUTASE IN ASCARIS-SUUM
    SANCHEZMORENO, M
    GARCIARUIZ, MA
    SANCHEZNAVAS, A
    MONTEOLIVA, M
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1989, 92 (04): : 737 - 740
  • [3] THE COPPER-ZINC SUPEROXIDE-DISMUTASE
    FEE, JA
    [J]. METAL IONS IN BIOLOGICAL SYSTEMS, 1981, 13 : 259 - 298
  • [4] COPPER AND COPPER-ZINC SUPEROXIDE-DISMUTASE
    VALENTINE, JS
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1983, 186 (AUG): : 229 - INOR
  • [5] SPECTROELECTROCHEMISTRY OF COPPER-ZINC SUPEROXIDE-DISMUTASE
    STCLAIR, CS
    GRAY, HB
    VALENTINE, JS
    [J]. INORGANIC CHEMISTRY, 1992, 31 (05) : 925 - 927
  • [6] REDESIGN OF COPPER-ZINC SUPEROXIDE-DISMUTASE
    ELLERBY, LM
    GRADEN, JA
    NISHIDA, CR
    VALENTINE, JS
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 208 : 183 - INOR
  • [7] Purification and characterization of iron superoxide dismutase and copper-zinc superoxide dismutase from Acanthamoeba castellanii
    Choi, DH
    Na, BK
    Seo, MS
    Song, HR
    Song, CY
    [J]. JOURNAL OF PARASITOLOGY, 2000, 86 (05) : 899 - 907
  • [8] PURIFICATION AND CHARACTERIZATION OF CANINE MANGANESE SUPEROXIDE-DISMUTASE AND ITS IMMUNOHISTOCHEMICAL LOCALIZATION IN CANINE HEART COMPARED WITH THAT OF COPPER-ZINC SUPEROXIDE-DISMUTASE
    IKEDA, K
    MATSUMI, S
    MAGARA, T
    NAKAGAWA, S
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1995, 27 (12): : 1257 - 1265
  • [9] THE AMINO-ACID-SEQUENCE OF COPPER-ZINC SUPEROXIDE-DISMUTASE FROM SWORDFISH LIVER COMPARISON OF COPPER-ZINC SUPEROXIDE-DISMUTASE SEQUENCES
    ROCHA, HA
    BANNISTER, WH
    BANNISTER, JV
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 145 (03): : 477 - 484
  • [10] METAL SITES OF COPPER-ZINC SUPEROXIDE-DISMUTASE
    BEEM, KM
    RICHARDSON, DC
    RAJAGOPALAN, KV
    [J]. BIOCHEMISTRY, 1977, 16 (09) : 1930 - 1936
12345