FLOW-INJECTION DETERMINATION OF 3-HYDROXYBUTYRATE IN SERUM WITH AN IMMOBILIZED 3-HYDROXYBUTYRATE DEHYDROGENASE REACTOR AND FLUORESCENCE DETECTION

被引：9
|
作者：
KIBA, N
KOEMADO, H
FURUSAWA, M
机构：
[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu
来源：
TALANTA | 1994年 / 41卷 / 09期
关键词：
D O I：
10.1016/0039-9140(94)E0056-W
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A flow-injection system with an immobilized enzyme reactor is proposed for the determination of 3-hydroxybutyrate. 3-Hydroxybutyrate dehydrogenase is immobilized on aminated poly(vinyl alcohol) beads and packed into a stainless-steel column (4 cm x 4 mm I.D.). Serum is diluted and filtered. Sample solution (20 mul) is injected into the carrier stream [4mM NAD+ in glycine buffer (pH 9.3)]. The NADH formed is detected at 465 nm (excitation at 340 nm). The calibration graph is linear for 0.7-500muM 3-hydroxybutyrate; the detection limit is 0.5muM.
引用
收藏
页码：1583 / 1586
页数：4
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