SEQUENCE OF THE GENE ENCODING AN ALKALINE SERINE-PROTEASE OF THERMOPHILIC BACILLUS-SMITHII

被引：4
|
作者：
MILANO, A
MANACHINI, PL
PARINI, C
RICCARDI, G
机构：
[1] UNIV PAVIA,DIPARTIMENTO GENET & MICROBIOL,I-27100 PAVIA,ITALY
[2] UNIV MILAN,DIPARTIMENTO SCI & TECHNOL ALIMENTARI & MICROBIOL,I-20133 MILAN,ITALY
来源：
GENE | 1994年 / 145卷 / 01期
关键词：
RECOMBINANT DNA; DNA SEQUENCING; PCR; INVERSE PCR;
D O I：
10.1016/0378-1119(94)90340-9
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The complete nucleotide sequence of the gene encoding an alkaline serine protease from Bacillus smithii has been determined. Degenerate oligodeoxyribonucleotide primers were used to prime the amplification of a 507-bp sequence of the gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction, using as template self-ligated DNA fragments. The deduced amino-acid sequence is compared to serine proteases from B. amyloliquefaciens, B. licheniformis, B. subtilis and Thermus aquaticus.
引用
收藏
页码：149 / 150
页数：2
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