DEVELOPMENT OF AN ALTERNATIVE METHOD FOR TESTING THE IMMUNOGENICITY OF DIPHTHERIA VACCINES

被引：5

作者：

MANGHI, MA ^{[1
]}

PASETTI, MF ^{[1
]}

BRERO, ML ^{[1
]}

DELUCHI, S ^{[1
]}

DIPAOLA, G ^{[1
]}

MATHET, V ^{[1
]}

ERIKSSON, PV ^{[1
]}

机构：

[1] INST NACL MICROBIOL DR CARLOS G MALBRAN,DEPT CONTRALOR,BUENOS AIRES,DF,ARGENTINA

来源：

VACCINE | 1995年 / 13卷 / 06期

关键词：

ANTI-DIPHTHERIA ANTIBODIES; DIPHTHERIA TOROID VACCINE; ELISA;

D O I：

10.1016/0264-410X(94)00047-Q

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immunogenicity of the diphtheria component of 73 commercial vaccines fr om Jive different manufacturers was rested by the toxin neutralization test (TNT) and the enzyme-linked immunosorbent assay (ELISA) developed in our laboratory. A comparison of the antibody levels measured by both assays showed a very good correlation (r=0.95, p < 0.001). The results suggest that the proposed ELISA is a reliable, simple and economical alternative to the TNT in guinea pigs. Also, the ELISA was found to measure IgG antibody levels as low as 5.5x10(-5) IU ml(-1). To evaluate the possibility of accelerating the active immunization during the activity test of vaccines an alternative schedule using one single human dose was assayed A very good correlation was observed between the IgG antibody response obtained with this schedule and with the traditional programme. Therefore, the cost and the time required to perform the activity test may be considerably reduced when both the vapid immunization schedule and the ELISA are used.

引用

页码：597 / 601

页数：5

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