INVESTIGATION AND IDENTIFICATION SALMO TRUTTA CASPIUS BY THE GROWTH HORMONE TYPE 1 (GH1) GENE ANALOGUE

The genetic growth hormone GH) relationships between several salmon and Salmo trutta caspius were analyzed by the sequencing technique. The GH gene is concluded by duplicated, isophorms determined as GH1 and GH2. In salmons GH gene 1 has 5 exons and 6 introns. At this study we have isolated and characterized GH1 gene containing 3 1 kbp. In related to, have designed one pair of primers from full length of GH gene was reported in the NCBI Network system (Accession nos. AY614010 ([RYYNANEN and PIUMMER et al, 2004]) and X61938([MALE et.al, 1992]), DNA genomic extracted by absorption on the glass (kit) method, we performed PCR polymorphism and the sequences of the gene were also identified, annotated and compared to each other in their coding regions and function them. We show that regions have compared between other populations of salmons by NCBI Network program. These results also were shown that high homology between Growth hormone gene in salmo trutta caspius and other salmons was recorded (approximately 97%).