CLONING AND EXPRESSION OF BACILLUS-STEAROTHERMOPHILUS NEUTRAL PROTEINASE GENE IN BACILLUS-SUBTILIS

被引：0
|
作者：
SIDORENKOV, IN
NOSOVSKAYA, EA
KOSTROV, SV
机构：
来源：
MOLECULAR BIOLOGY | 1995年 / 29卷 / 03期
关键词：
BACILLI; METALLOPROTEINASES; CLONING; EXPRESSION; THERMOSTABILITY;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A gene library of the thermophilic bacterium Bacillus stearothermophilus was made in a plasmid vector, and the gene of thermostable metalloproteinase was cloned. Restriction mapping proved it to be close to the B. stearothermophilus metalloproteinase T gene cloned earlier. The cloned expressed in B. subtilis, and its protein product was characterized in terms of heat stability and temperature optimum.
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页码：286 / 288
页数：3
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