LABELING OF HISTONE H5 AND ITS INTERACTION WITH DNA .1. HISTONE H5 LABELING WITH FLUORESCEIN ISOTHIOCYANATE

被引：9

作者：

FAVAZZA, M ^{[1
]}

LERHO, M ^{[1
]}

HOUSSIER, C ^{[1
]}

机构：

[1] UNIV LIEGE SART TILMAN B6,CHIM MACROMOLEC & CHIM PHYS LAB,B-4000 LIEGE,BELGIUM

来源：

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 1990年 / 7卷 / 06期

关键词：

D O I：

10.1080/07391102.1990.10508566

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histone H5 has been labelled with fluorescein isothiocyanate (FITC) with particular attention to the reaction conditions (pH, reaction time and input FITC/H5 molar ratio) and to the complete elimination of non-covalently bound dye. We preferred to use reaction conditions which yielded non-specific uniform labelling rather than specific a-NH2 terminal labelling, in order to obtain higher sensitivity in further studies dealing with the detection of perturbation at the binding sites of H5 on DNA. © Taylor & Francis Group, LLC.

引用

页码：1291 / 1300

页数：10

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