CHARACTERIZATION AND GENERATION OF ESCHERICHIA-COLI ADENYLATE-CYCLASE DELETION MUTANTS

被引：44
|
作者：
SHAH, S [1 ]
PETERKOFSKY, A [1 ]
机构：
[1] NHLBI,BIOCHEM GENET LAB,BETHESDA,MD 20892
来源：
JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 10期
关键词：
D O I：
10.1128/JB.173.10.3238-3242.1991
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Escherichia coli DELTA-cya-283 is a 75-bp in-frame deletion overlapping the 5' end of DELTA-cya-854; DELTA-cya-201 is a 41-bp frameshift deletion overlapping the 3' end of DELTA-cya-854. Sequence repeats were found at the boundaries of DELTA-cya-283 and DELTA-cya-201, suggesting a mechanism for deletion formation. Recombinant DNA procedures were used to construct a strain in which the total cya structural gene in the chromosome was replaced by the kanamycin resistance gene.
引用
收藏
页码：3238 / 3242
页数：5
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