SILICA-BASED SUPPORTS FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF BIOPOLYMERS USING NONDENATURING CONDITIONS

被引：7
|
作者：
FREISER, HH
GOODING, KM
机构：
[1] SynChrom, Inc., Lafayette, IN 47902
来源：
JOURNAL OF CHROMATOGRAPHY | 1991年 / 544卷 / 1-2期
关键词：
D O I：
10.1016/S0021-9673(01)83982-0
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Silica-based supports have been used successfully as media for high-performance liquid chromatography (HPLC) of proteins. Their characteristics of particle diameter, pore diameter, rigidity and suitability for derivatization have made them well accepted for all relevant HPLC techniques. In this article, physical properties and operating parameters of silica-based supports for those HPLC modes which render proteins intact and biologically active will be discussed.
引用
收藏
页码：125 / 135
页数：11
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