POLYMERASE CHAIN-REACTION DETECTION OF PLASMODIUM-FALCIPARUM IN MOSQUITOS

被引：37
|
作者：
TASSANAKAJON, A
BOONSAENG, V
WILAIRAT, P
PANYIM, S
机构：
[1] MAHIDOL UNIV,FAC SCI,DEPT BIOCHEM,BANGKOK 10400,THAILAND
[2] SRINAKHARINWIROT UNIV,FAC MED,DEPT BIOCHEM,BANGKOK,THAILAND
来源：
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1993年 / 87卷 / 03期
关键词：
D O I：
10.1016/0035-9203(93)90124-9
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections.
引用
收藏
页码：273 / 275
页数：3
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