BNC1促进肺鳞状细胞癌细胞迁移和侵袭

被引：0

作者：

张珑耀 ^{[1
]}

曾泷锦 ^{[2
]}

郑林鹏 ^{[1
]}

林俸林 ^{[1
]}

蔡定钦 ^{[1
]}

蔡萍 ^{[1
]}

孙建国 ^{[1
]}

机构：

[1] 陆军军医大学(第三军医大学)第二附属医院肿瘤科

[2] 不详

来源：

陆军军医大学学报 | 2024年 / 46卷 / 07期

关键词：

碱性核蛋白1; E-钙粘蛋白; 肺鳞状细胞癌; 迁移; 侵袭;

D O I：

10.16016/j.2097-0927.202312131

中图分类号：

R734.2 [肺肿瘤];

学科分类号：

摘要：

目的探索碱性核蛋白1(basonuclin 1,BNC1)基因对肺鳞状细胞癌细胞迁移和侵袭能力的影响及其作用机制。方法利用生物信息学分析在线数据库中BNC1基因在肺鳞状细胞癌患者中的表达规律及对生存的影响；采用免疫组织化学对组织芯片进行染色并分析BNC1的蛋白水平；在癌症细胞系百科全书(Cancer Cell Line Encyclopedia, CCLE)数据库中筛选BNC1高表达和低表达的肺鳞状细胞癌细胞系；对敲低和过表达的肺鳞状细胞癌细胞H226、SK-MES1进行划痕愈合实验、Transwell迁移和侵袭实验，检测细胞的迁移与侵袭能力的变化；采用RT-qPCR验证10个文献来源的有关迁移与侵袭高度相关的基因；采用Western blot对筛选的调控基因进一步验证。结果生物信息学分析发现BNC1高表达于肺鳞状细胞癌组织且与较低生存率相关(P=0.047);肺鳞状细胞癌组织中BNC1的表达高于癌旁组织(P<0.05);H226细胞系BNC1表达较高，而SK-MES1细胞系BNC1表达较低；敲低BNC1能够抑制肺鳞状细胞癌细胞的迁移和侵袭(P<0.05),过表达BNC1可增强肺鳞状细胞癌细胞的迁移和侵袭(P<0.05);在mRNA层面筛选BNC1下游调控分子，发现E-钙粘蛋白(E-cadherin,CDH1)在BNC1过表达时减低(P<0.05),在BNC1敲低时升高(P<0.05),Western blot验证结果与mRNA表达一致(P<0.05)。结论 BNC1可能是肺鳞状细胞癌患者的预后不良基因，BNC1基因可能通过下调CDH1的表达，促进肺鳞状细胞癌细胞的迁移和侵袭。

引用

页码：678 / 687

页数：10

共 27 条

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