Fine mapping and cloning of the sterility gene Bra2Ms in nonheading Chinese cabbage(Brassica rapa ssp. chinensis)

Theapplicationofamale-sterilelineisanidealapproachforhybridseedproductioninnon-headingChinese cabbage (Brassica rapa ssp. chinensis).However, the molecular mechanisms underlying male sterility in B. rapa arestilllargelyunclear.WepreviouslyobtainedthenaturalmalesterilelineWS24-3ofnon-headingChinese cabbage and located the male sterile locus, Bra2Ms, on the A2 chromosome.Cytological observations revealed thatthemalesterilityofWS24-3resultedfromdisruptionofthemeiosisprocessduringpollenformation.Fine mappingofBra2Msdelimitedthelocuswithinaphysicaldistanceofabout129kbonthe A2chromosomeofB.rapa.TheBra039753 gene encodes a plant homeodomain (PHD)-finger protein and is considered a potential candidate gene for Bra2Ms.Bra039753 was significantly downregulated in sterile line WS24-3 compared to the fertile line at the meiotic anther stage.Sequence analysis of Bra039753 identified a 369 bp fragment insertion in the first exon in male sterile plants, which led to an amino acid insertion in the Bra039753 protein.In addition, the369 bp fragment insertion was found to cosegregate with the male sterility trait.This study identified a novel locus related to male sterility in non-heading Chinese cabbage, and the molecular marker obtained in this study will be beneficial for the marker-assisted selection of excellent sterile lines in non-heading Chinese cabbage and other Brassica crops.