Electrospun PVA–Dextran Nanofibrous Scaffolds for Acceleration of Topical Wound Healing: Nanofiber Optimization, Characterization and In Vitro Assessment

Electrospun polyvinyl alcohol–dextran (PVA–Dex)-based nanofibers (NFs) are explored as a novel class of bioactive injury dressing materials, which have an essential role for topical injury mending. Sodium ampicillin-loaded citric acid-cross-linked PVA–Dex NFs were fabricated by electrospinner for wound recuperating purposes. Results revealed that PVA (10%)–dextran (10%) cross-linked with 5% citric acid (CA) was chosen as an optimized condition for obtaining non-beaded and morphological accepted nanofibers. Altered concentrations of CA as cross-linker progressively enhanced significantly the mechanical/thermal stability and wettability-proof of NFs scaffolds, compared to un-cross-linked (PVA–Dex) scaffolds. Meanwhile, swelling (%), protein adsorption and released ampicillin of NFs decreased dramatically with the increase in the CA concentration, and conversely enhanced with increasing dextran concentrations. Interestingly, resultant PVA–Dex NFs with high concentrations of dextran promoted the proliferation of HFB-4 cells in a high concentration-dependent manner and high antimicrobial activity behavior, compared to NFs containing high concentrations of CA cross-linker after 24 and 48 h of cell exposure. Notably, all fabricated NFs have remarked ability to accelerate the rate of in vitro wound gap closure (%) after treatment for 24 and 48 h, compared to control sample. However, reducing CA concentration in NFs showed the highest percentages of wound healing for scratched HFB-4 cells with clear observed healing process.