Changes in fatty acid composition of Stenotrophomonas maltophilia KB2 during co-metabolic degradation of monochlorophenols

被引:0
|
作者
Agnieszka Nowak
Izabela Greń
Agnieszka Mrozik
机构
[1] University of Silesia,Department of Biochemistry, Faculty of Biology and Environmental Protection
关键词
Co-metabolism; Fatty acid composition; Monochlorophenols; KB2;
D O I
暂无
中图分类号
学科分类号
摘要
The changes in the cellular fatty acid composition of Stenotrophomonas maltophilia KB2 during co-metabolic degradation of monochlorophenols in the presence of phenol as well as its adaptive mechanisms to these compounds were studied. It was found that bacteria were capable of degrading 4-chlorophenol (4-CP) completely in the presence of phenol, while 2-chlorophenol (2-CP) and 3-chlorophenol (3-CP) they degraded partially. The analysis of the fatty acid profiles indicated that adaptive mechanisms of bacteria depended on earlier exposure to phenol, which isomer they degraded, and on incubation time. In bacteria unexposed to phenol the permeability and structure of their membranes could be modified through the increase of hydroxylated and cyclopropane fatty acids, and straight-chain and hydroxylated fatty acids under 2-CP, 3-CP and 4-CP exposure, respectively. In the exposed cells, regardless of the isomer they degraded, the most important changes were connected with the increase of the contribution of branched fatty acid on day 4 and the content of hydroxylated fatty acids on day 7. The changes, particularly in the proportion of branched fatty acids, could be a good indicator for assessing the progress of the degradation of monochlorophenols by S. maltophilia KB2. In comparison, in phenol-degrading cells the increase of cyclopropane and straight-chain fatty acid content was established. These findings indicated the degradative potential of the tested strain towards the co-metabolic degradation of persistent chlorophenols, and extended the current knowledge about the adaptive mechanisms of these bacteria to such chemicals.
引用
收藏
相关论文
共 27 条
  • [1] Changes in fatty acid composition of Stenotrophomonas maltophilia KB2 during co-metabolic degradation of monochlorophenols
    Nowak, Agnieszka
    Gren, Izabela
    Mrozik, Agnieszka
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2016, 32 (12):
  • [2] Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation
    Wojcieszynska, Danuta
    Klamka, Judyta
    Marchlewicz, Ariel
    Potocka, Izabela
    Zur-Pinska, Joanna
    Guzik, Urszula
    [J]. MOLECULES, 2022, 27 (18):
  • [3] Facilitation of Co-Metabolic Transformation and Degradation of Monochlorophenols by Pseudomonas sp CF600 and Changes in Its Fatty Acid Composition
    Nowak, Agnieszka
    Mrozik, Agnieszka
    [J]. WATER AIR AND SOIL POLLUTION, 2016, 227 (03):
  • [4] Facilitation of Co-Metabolic Transformation and Degradation of Monochlorophenols by Pseudomonas sp. CF600 and Changes in Its Fatty Acid Composition
    Agnieszka Nowak
    Agnieszka Mrozik
    [J]. Water, Air, & Soil Pollution, 2016, 227
  • [5] The potential of Stenotrophomonas maltophilia KB2 for phenol degradation under exposure to heavy metal
    Gaszczak, Agnieszka
    Szczyrba, Elzbieta
    Szczotka, Anna
    [J]. CHEMICAL AND PROCESS ENGINEERING-NEW FRONTIERS, 2024, 45 (02):
  • [6] Implications of Bacterial Adaptation to Phenol Degradation under Suboptimal Culture Conditions Involving Stenotrophomonas maltophilia KB2 and Pseudomonas moorei KB4
    Nowak, Agnieszka
    Wasilkowski, Daniel
    Mrozik, Agnieszka
    [J]. WATER, 2022, 14 (18)
  • [7] Degradation of 4-chlorophenol and microbial diversity in soil inoculated with single Pseudomonas sp CF600 and Stenotrophomonas maltophilia KB2
    Nowak, Agnieszka
    Mrozik, Agnieszka
    [J]. JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2018, 215 : 216 - 229
  • [8] Degradation Potential of Protocatechuate 3,4-Dioxygenase from Crude Extract of Stenotrophomonas maltophilia Strain KB2 Immobilized in Calcium Alginate Hydrogels and on Glyoxyl Agarose
    Guzik, Urszula
    Hupert-Kocurek, Katarzyna
    Krysiak, Marta
    Wojcieszynska, Danuta
    [J]. BIOMED RESEARCH INTERNATIONAL, 2014, 2014
  • [9] Protocatechuate 3,4-Dioxygenase: A Wide Substrate Specificity Enzyme Isolated from Stenotrophomonas maltophilia KB2 as a Useful Tool in Aromatic Acid Biodegradation
    Guzik, Urszula
    Hupert-Kocurek, Katarzyna
    Sitnik, Malgorzata
    Wojcieszynska, Danuta
    [J]. JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 24 (03) : 150 - 160
  • [10] High activity catechol 1,2-dioxygenase from Stenotrophomonas maltophilia strain KB2 as a useful tool in cis,cis-muconic acid production
    Guzik, Urszula
    Hupert-Kocurek, Katarzyna
    Sitnik, Malgorzata
    Wojcieszynska, Danuta
    [J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2013, 103 (06): : 1297 - 1307