Analysis of gene sequences for the nucleocapsid protein from Tomato spotted wilt virus for promoting RNA-mediated cross-protection using the Potato virus X vector system

被引:3
|
作者
Sonoda S. [1 ]
Tsumuki H. [1 ]
机构
[1] Research Institute for Bioresources, Okayama University, Kurashiki
关键词
Cross-protection; Nicotiana benthamiana; RNA silencing; Tospovirus; Virus resistance;
D O I
10.1007/s10327-003-0116-1
中图分类号
学科分类号
摘要
Virus interactions between Tomato spotted wilt virus (TSWV) and Potato virus X (PVX) containing the nucleocapsid protein (N) gene sequences were examined to evaluate the capacity of the N gene sequences from TSWV to promote RNA-mediated cross-protection. Plants simultaneously inoculated with TSWV and PVX containing the 3′ 96bp of the N gene were highly resistant to TSWV infection, whereas no such resistance was observed in plants inoculated with TSWV and PVX containing the 5′ 96 bp. These results suggest that the 3′ portion of the N gene has a higher capacity for promoting RNA-mediated cross-protection of TSWV.
引用
收藏
页码:239 / 242
页数:3
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