Rosmarinic acid production by Lavandula vera MM cell suspension culture: nitrogen effect

被引:0
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作者
M. Ilieva
A. Pavlov
机构
[1] Bulgarian Academy of Sciences,Institute of Microbiology, Laboratory of Biotechnology and Industrial Microbiology
关键词
Cell culture; Lamiaceae; Lavandula vera MM; nitrogen effect; rosmarinic acid;
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摘要
The growth of Lavandula vera MM cell suspension and the biosynthesis of rosmarinic acid (RA) were followed during its cultivation in Linsmayer–Skoog media, containing different concentrations of ammonium and nitrate ions. The results showed that cultivation in a medium with 0.09 g ammonium ions/l (1/4 of standard medium) ensured intensive growth (16 g dry biomass/l) and enhanced biosynthesis of RA (15 mg/g dry biomass). Cultivation of L. vera MM cell culture in a medium with 1.2-fold concentration of nitrate ions led to accumulation of 11 mg RA/g dry biomass which was twice as much as in the standard Linsmayer–Skoog medium.
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页码:711 / 714
页数:3
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