Characterization of DNA immobilized on electrochemically prepared conducting polypyrrole-polyvinyl sulfonate films

被引：0
|
作者：
Anamika Gambhir
Manju Gerard
Shailesh K. Jain
B. D. Malhotra
机构：
[1] National Physical Laboratory,Biomolecular Electronics and Conducting Polymer Research Group
来源：
Applied Biochemistry and Biotechnology | 2001年 / 96卷
关键词：
DNA biosensor; immobilization; conducting polymers; polypyrrole/polyvinyl sulfonate;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The article describes the adsorption characteristics of DNA onto electrochemically generated polypyrrole-polyvinyl sulfonate (PPY-PVS) films obtained as a function of pH. Adsorption on PPY doped with an anion proceeds by anion exchange, and since DNA possesses a fixed negative charge owing to PO4−, it favors a very strong binding displacing PVS with favorable energetic interactions. Characterization of adsorbed DNA onto the PPY-PVS films was carried out by ultraviolet-visible, Fourier transform infrared spectroscopy, and cyclic voltammetric studies.
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页码：313 / 319
页数：6
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