Real-time quantitative assay of telomerase activity using the duplex scorpion primer

被引：0
|
作者：
Yanping Huang
Deming Kong
Yi Yang
Ruifang Niu
Hanxi Shen
Huaifeng Mi
机构：
[1] Nankai University,State Key Lab of Functional Polymer Materials for Adsorption and Separation, Chemical School
[2] Nankai University,Medical School
[3] Cancer Hospital,Oncology Center Laboratory
[4] Tianjin Medical University,College of Pharmacy
来源：
Biotechnology Letters | 2004年 / 26卷
关键词：
duplex scorpion primer; real-time quantitative PCR; telomerase activity; TRAP assay;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpion primer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Using this method, linearity from 10 to 104 cells expressing telomerase activity could be obtained (R2=0.994). The requirement of post-PCR steps is thus obviated.
引用
收藏
页码：891 / 895
页数：4
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